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Functional interaction between RNA helicase II/Guα and ribosomal protein L4
Author(s) -
Yang Hushan,
Henning Dale,
Valdez Benigno C.
Publication year - 2005
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2005.04811.x
Subject(s) - rna helicase a , rna , microbiology and biotechnology , biology , ribosome , ribosomal rna , ribosomal protein , small interfering rna , eukaryotic small ribosomal subunit , helicase , genetics , gene
RNA helicase II/Guα is a multifunctional nucleolar protein involved in ribosomal RNA processing in Xenopus laevis oocytes and mammalian cells. Downregulation of Guα using small interfering RNA (siRNA) in HeLa cells resulted in 80% inhibition of both 18S and 28S rRNA production. The mechanisms underlying this effect remain unclear. Here we show that in mammalian cells, Guα physically interacts with ribosomal protein L4 (RPL4), a component of 60S ribosome large subunit. The ATPase activity of Guα is important for this interaction and is also necessary for the function of Guα in the production of both 18S and 28S rRNAs. Knocking down RPL4 expression using siRNA in mouse LAP3 cells inhibits the production of 47/45S, 32S, 28S, and 18S rRNAs. This inhibition is reversed by exogenous expression of wild‐type human RPL4 protein but not the mutant form lacking Guα‐interacting motif. These observations have suggested that the function of Guα in rRNA processing is at least partially dependent on its ability to interact with RPL4.