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Peroxiredoxin II functions as a signal terminator for H 2 O 2 ‐activated phospholipase D1
Author(s) -
Xiao Nianzhou,
Du Guangwei,
Frohman Michael A.
Publication year - 2005
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2005.04809.x
Subject(s) - phospholipase d , stimulation , intracellular , signal transduction , biology , microbiology and biotechnology , biochemistry , endocrinology
Phospholipase D1 (PLD1) is a signal‐transduction regulated enzyme which regulates several cell intrinsic processes including activation of NAPDH oxidase, which elevates intracellular H 2 O 2 . Several proteins have been reported to interact with PLD1 in resting cells. We sought to identify proteins that interact with PLD1 after phorbol 12‐myristate 13‐acetate (PMA) stimulation. A novel interaction with peroxiredoxin II (PrxII), an enzyme that eliminates cellular H 2 O 2 , which is a known stimulator of PLD1, was identified by PLD1‐affinity pull‐down and MS. PMA stimulation was confirmed to promote physical interaction between PLD1 and PrxII and to cause PLD1 and PrxII to colocalize subcellularly. Functional significance of the interaction was suggested by the observation that over‐expression of PrxII specifically reduces the response of PLD1 to stimulation by H 2 O 2 . These results indicate that PrxII may have a signal‐terminating role for PLD1 by being recruited to sites containing activated PLD1 after cellular stimulation involving production of H 2 O 2 .