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Modular usage of the HLA‐DRA promoter in extra‐hematopoietic and hematopoietic cell types of transgenic mice
Author(s) -
Giorda Ezio,
Sibilio Leonardo,
Martayan Aline,
Feriotto Giordana,
Bianchi Nicoletta,
Mischiati Carlo,
Di Rosa Francesca,
Pozzi Laura,
Gambari Roberto,
Giacomini Patrizio
Publication year - 2005
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2005.04740.x
Subject(s) - biology , transgene , haematopoiesis , microbiology and biotechnology , gene , major histocompatibility complex , mhc class i , stem cell , genetics
Class II MHC genes (for example, the human HLA‐DRA gene) are expressed at the cell surface in many professional and nonprofessional antigen‐presenting cells in a variety of anatomical locations. Here, we report about 13 mouse transgenic lines (11 of which have not been previously described) generated with four distinct sets of DRA transgenes carrying progressive, informative 5′ and 3′ deletions. DRA expression was assessed in B lymphocytes, dendritic cells, macrophages, and extra‐hematopoietic cells (particularly kidney epithelial cells). A compact transcriptional unit was identified that efficiently directs DRA expression [both constitutive and interferon (IFN)‐γ induced] in extra‐hematopoietic tissues and dendritic cells. It extends from position −266 upstream of the transcription initiation site to position +119 downstream of the last DRA exon. The same fragment, however, did not efficiently direct IFN‐γ‐induced DRA expression in macrophages, that required additional 5′ sequences. Thus, IFN‐γ uses distinct promoter segments and mechanisms to up‐regulate class II in different cell lineages. In contrast to previous results in transgenic mice expressing murine class II transgenes, we were unable to generate reproducible patterns of HLA‐DRA expression in B cells.