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Dietary exposure to low doses of bisphenol A: Effects on reproduction and development in two generations of C57BL/6J mice
Author(s) -
Kobayashi Kenichi,
Ohtani Katsumi,
Kubota Hisayo,
Miyagawa Muneyuki
Publication year - 2010
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.2010.00279.x
Subject(s) - reproduction , medicine , sperm , endocrinology , epididymis , biology , gestation , anogenital distance , bisphenol a , ovary , pregnancy , andrology , fetus , in utero , chemistry , ecology , genetics , organic chemistry , epoxy
The present study was conducted to examine the effects of low‐dose exposure to bisphenol A on reproduction and development in two generations of mice. Pregnant female C57BL/6J mice (F 0 ) were fed a diet containing low doses of bisphenol A (0, 0.33, 3.3, or 33 ppm) from gestational day 6 through postnatal day 22, and the weanlings (F 1 and F 2 ) from each F 0 and F 1 dam group, respectively, were also fed these same concentrations of bisphenol A ad libitum until sacrifice. There were no treatment‐related changes in body weight, body weight gain, food consumption, gestation length, or the number of live births on postnatal day 1 in F 0 dams between the control group and bisphenol A groups. Sex ratio and viability were similar in all F 1 pups. No treatment‐related changes were observed in body weight, food consumption, developmental parameters, anogenital distance, or weight of any of the organs (liver, kidney, heart, spleen, thymus, testis, ovary, or uterus) in F 1 and F 2 adults in either sex. The epididymis weight was slightly higher with 0.33 and 3.3 ppm in F 1 males, but this slight increase was neither dose dependent nor seen across generations. There were no treatment‐related effects of bisphenol A on cauda epididymal sperm count or sperm motility in F 1 or F 2 males. These findings indicate that dietary exposure to bisphenol A between 0.33 and 33 ppm does not adversely affect reproduction or development as assessed in two generations of mice.