Premium
Association of tenascin‐W expression with mineralization in mouse calvarial development
Author(s) -
Mikura Ayako,
Okuhara Shigeru,
Saito Masahiro,
Ota Masato,
Ueda Koichi,
Iseki Sachiko
Publication year - 2009
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.2009.00227.x
Subject(s) - tenascin , mineralization (soil science) , biology , microbiology and biotechnology , extracellular matrix , ecology , fibronectin , soil water
Tenascin‐W is a tenascin family member that forms part of a complex extracellular matrix, and previous studies have suggested its association with osteogenesis. In the present study we investigated the roles of tenascin‐W in osteogenesis. We found that tenascin‐W is expressed in osteoblasts at the edge of the developing bone domain prior to mineralization in mouse fetuses. Expression of tenascin‐W was induced during the course of mineralization of the Kusa‐A1 osteoblast cell line. In the interfrontal suture of postnatal mice, the anterior portion remains patent and the posterior portion closes by 4 weeks of age. Tenascin‐W expression was downregulated at 1 week of age in the posterior frontal suture, whereas in the anterior suture, expression was maintained until the mice reached 4 weeks of age. Fibroblast growth factor 2 (FGF2)‐bead application to the mouse fetal skull by ex‐utero surgery accelerated osteoblast differentiation, but inhibited mineralization with a downregulation of tenascin‐W expression. These results suggest that tenascin‐W is involved in osteoblast maturation (i.e. mineralization).