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Analysis of polyploid cells in mouse embryonic cells cultured under diabetic conditions
Author(s) -
Tatewaki Reiko,
Kagohashi Yukiko,
Otani Hiroki
Publication year - 2006
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.2006.00118.x
Subject(s) - group b , embryonic stem cell , embryo , apoptosis , polyploid , biology , group a , ketone bodies , andrology , endocrinology , l glucose , diabetes mellitus , medicine , microbiology and biotechnology , biochemistry , ploidy , metabolism , gene , islet
To clarify the cytogenetic effects of glucose and ketone bodies on the pathogenesis of diabetes‐associated congenital anomalies, we cultured cells from gestation‐day‐8 ICR mouse embryos under the diabetic condition. Cells were cultured in the medium with glucose (300 mg/dL) plus DL‐2‐hydroxybutyric acid (32 mM) (G + B group), glucose alone (G group), or neither of them (C group) for 5 days. At the end of the culture, cells were analyzed for the chromosomes. After 3–4 days culture, when the living cells grew into a mono‐layered sheet, cells floating in the medium were observed and showed morphological features of apoptosis. Ratio of the floating cells was significantly higher in the G + B group than in the G or C group ( P < 0.05), suggesting the deleterious effect of glucose and ketone body. Polyploidy was observed in the cultured cells more frequently in the G + B group (64.1%) than in the G group (49.0%), which was higher than the C group (20.5%) (G + B vs G: P < 0.05, G vs C: P < 0.001). The higher ratio of the polyploidy, but not of the aneuploidy, in the G + B and G groups suggested the specific effect of glucose and ketone body for inducing polyploidy. These results suggest that diabetic condition causes polyploidy in cultured embryonic cells.