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Effects of pure 2,4‐dichlorophenoxyacetic acid on cultured rat embryos
Author(s) -
Sameshima Kiyoko,
Kobae Hidehiko,
Fofana Djibril,
Yoshidome Kouichi,
Nishi Junichiro,
Miyata Koichiro
Publication year - 2004
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.2004.00014.x
Subject(s) - 2,4 dichlorophenoxyacetic acid , embryo , andrology , chemistry , biology , microbiology and biotechnology , botany , medicine
  2,4‐dichlorophenoxyacetic acid (2,4‐D), a plant growth regulator, has been used worldwide as a herbicide. Previously we evaluated the prenatal developmental effects of 2,4‐D by feeding it to pregnant rats and found that it is maternally toxic and embryolethal, and it induces urogenital malformations in rat fetuses. In the study presented here, we investigated the effects of pure 2,4‐D on rat embryos in whole embryo culture. Rat embryos on day 9.5 of gestation were cultured for 48 h at several concentration levels with pure 2,4‐D (50–500 µg/mL). 2,4‐D caused a concentration‐related increase in the incidence of each malformation. Significant decreases in the number of somites were observed at a concentration of 100 µg/mL or more. At the concentration of 100 µg/mL, there was normal yolk sac circulation. This result suggests that 2,4‐D has a detrimental effect on somite development and directly damages developing embryos.

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