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Eyelid and Pinnal Development after Maternal Treatment with Cortisone Acetate in NC‐eob Mouse Fetuses with a Genetically Determined Open‐Eyelid Malformation
Author(s) -
FUJII Sakiko,
KANEDA Masahiro,
TERAMOTO Shoji
Publication year - 1995
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.1995.tb00958.x
Subject(s) - eyelid , fetus , gestation , medicine , endocrinology , pregnancy , biology , surgery , genetics
NC‐eob mice are mutants having open eyelids at birth with complete penetrance. The present study was aimed at investigating the possible effects of cortisone acetate (CA) on the eyelid closure of NC‐eob mice. For comparison, fusion of pinnae to the scalp was also investigated. NC strain mice were used as a control. Females were given daily subcutaneous doses of 0, 20, 40 or 80 mg/kg of CA on days 13 to 15 of gestation. Live fetuses were removed from the uteri on day 16 or 18 of gestation. Day‐18 fetuses were weighed along with the placenta and examined for external abnormalities. Day‐16 fetuses were fixed in Bouin's fluid and examined for the developmental stages of eyelid closure and pinnal fusion. In day‐18 NC and NC‐eob fetuses, decreases in body weight and placental weight and an increase in the incidence of cleft palate were observed in a dose‐related manner. However, no eyelid closure occurred in any NC‐eob fetuses. In day‐16 NC‐eob fetuses, although slight enhancement of the eyelid growth and epidermal differentiation was seen, eyelid openings remained wide. Clear accelerating effects of CA treatment were noted for the pinnal fusion. In CA treated NC fetuses, both eyelid closure and pinnal fusion were clearly accelerated. These results indicate that the effects of CA on the eyelid development in NC‐eob fetuses are insufficient for closing eyelids.