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Effects of Serum to Cushion Mesenchymal Cell Migration of the Developing Chick Heart in Vitro
Author(s) -
SUMIDA Hiroshi,
NAKAMURA Harukazu,
ISHITOU Yukiko,
YASUDA Mineo
Publication year - 1995
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.1995.tb00612.x
Subject(s) - mesenchymal stem cell , in vitro , cushion , andrology , anatomy , cell migration , embryo , atrioventricular valve , heart development , biology , chemistry , microbiology and biotechnology , medicine , embryonic stem cell , biochemistry , mechanical engineering , ventricle , gene , engineering
Summary Migration of cardiac mesenchymal cells of chick embryos was examined in vitro . The conotruncal cushion and atrioventricular cushion cells of 6‐day‐old chick embryos were cultured on collagen gel in Dulbecco's modified Eagle's medium with chicken serum of calf serum, or no serum. After 48 hr of incubation, the culture was fixed with 4% formaldehyde, and photographed. The migration area of mesenchymal cells spread from the transplanted tissue was measured by digitizing the photographs. Then, the migration distance of mesenchymal cells was computed from the area with a personal computer. Mesenchymal cells cultured with chicken serum migrated a significantly longer distance than those with calf serum or no serum. Under the designed conditions, the migration distance of the mesenchymal cells of the conotruncal cushion was longer than that of the atrioventricular cushion. These results show that certain factor(s) in serum stimulates migration activity of cardiac mesenchymal cells, but the mammalian factor(s) is not cross‐reactive to cardiac mesenchymal cells of birds. Different effects of the serum from different species on the migration activity of chick cardiac mesenchymal cells were shown in the present results. At the same time, it was revealed that migration activity of mesenchymal cells of the conotruncal cushion is different from that of the atrioventricular cushion.