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Simple Methods for Objective Assessment of Sperm Viability and Motility with MTT Assay and Sperm Quality Analyzer (SQA) in Rats
Author(s) -
HARA Hiroaki,
TAKAI Ryou,
TANAKA Naomi,
MIZOGUCHI Keiji,
TSUNO Tatsuya,
IGARASHI Shinichi,
USAMI Masayoshi
Publication year - 1995
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.1995.tb00314.x
Subject(s) - sperm , andrology , sperm motility , semen , motility , mtt assay , semen quality , semen analysis , chemistry , sperm quality , biology , chromatography , medicine , infertility , in vitro , biochemistry , pregnancy , genetics
To confirm the applicability of MTT assay and sperm quality analyzer (SQA) to the examination of sperm viability and motility in rats, epididymal spermatozoa derived from male rats (Slc:SD) treated with nitrobenzene at a dose of 60 mg/kg/day for 16 days were examined by these methods. Epididymal fluid was suspended in Dulbecco's modified Eagle's medium containing 25 mM fructose in ratio of 1:125. Sperm concentrations in the epididymal fluid were 2.48 ± 0.55 (× 10 6 /μl, mean ± SD, n = 8) in the control and 2.20 ± 0.54 in the nitrobenzene group, and did not significantly differ between the groups. Both absorbance unit of the MTT assay (1.497 ± 0.406 vs. 1.084 ± 0.350) and SMI (sperm motility index) value of the SQA (204.0 ± 15.3 vs. 182.5 ± 21.6) were significantly decreased in nitrobenzene group. The results suggest that the examinations of sperm viability and motility with MTT assay and SQA are available for the reproductive toxicology in rats.