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Development of Rabbit Whole Embryo Culture during Organogenesis
Author(s) -
NAYA Masato,
KITO Yoshie,
ETO Kazuhiro,
DEGUCHI Takashi
Publication year - 1991
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.1991.tb00760.x
Subject(s) - embryo , yolk sac , embryo culture , andrology , biology , teratology , organogenesis , gestation , rabbit (cipher) , in vivo , embryogenesis , new zealand white rabbit , pregnancy , medicine , microbiology and biotechnology , genetics , statistics , mathematics , gene
The rodent embryo culture has widely been spread as a technique in teratogenicity studies. However, the whole embryo culture of rabbits has not been developed. Pregnant rabbits are usually used in a teratogenicity test as a non‐rodent animal to examine the species specificity. Rabbit embryos of the Japanese White strain on day 10 of gestation (the time of copulation = day 0) were cultured at 38°C for 48 hours in 100% rabbit serum. The 35 ml bottles with 5 ml serum were gassed with 95% 02 and 5%70 CO2 continuously. The yolk sac and amniotic sac were opened 24 hours after the beginning of the culture. At the end of the culture, embryos were examined for somite number, as well as protein content and crown‐rump length. Embryos cultured for 48 hours from day 10 seemed to be well developed as well as in vivo embryos. These results showed that rabbit embryo culture became a useful system for the study of reproductive and developmental toxicology, teratology and developmental biology.