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The Interaction of Oligosyndactyly ( Os ) Gene and 5‐Fluorouracil (5‐FU) in the Manifestation of Digital Malformations in Mice
Author(s) -
IMAMURA Takakazu
Publication year - 1990
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.1990.tb00500.x
Subject(s) - fetus , gestation , fluorouracil , teratology , medicine , endocrinology , biology , pregnancy , andrology , chemotherapy , genetics
The heterozygotes of Oligosyndactyly ( Os ) gene manifest syndactyly on fore‐and hindfeet and sometimes axial oligodactyly in mice. 5‐Fluorouracil (5‐FU) is known to produce various types of digital malformations in mice. The present experiment was designed to examine possible interactions of Os gene and 5‐FU in the induction of digital malformations in mice. Female Slc:ICR mice were mated with F1 males ( Os /+ or +/+) which were obtained from the cross between female ICR and male C57BL‐ Os /+ mice. Pregnant females were treated with a single intraperitoneal injection of teratogenic dose (20 mg/kg) or marginal teratogenic dose (10, 7.5 and 15 mg/kg) on day 9, 10 and 11 of gestation, respectively. On day 17 or 18 of gestation, fetuses of treated and non‐treated control groups were examined externally and in bone and cartilage double‐stained specimens. The teratogenic dose of 5‐FU increased the expressivity of Os gene and, in turn, Os gene caused an increased severity of 5‐FU‐induced postaxial oligodactyly. The interaction between Os gene and marginal teratogenic dose of 5‐FU varied according to the time of treatment. Increased expressivity of Os gene could be observed in the groups treated with the marginal teratogenic dose of 5‐FU on day 10 and day 11.