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Bone‐Staining Technique for Fetal Rat Specimens without Skinning and Removing Adipose Tissue
Author(s) -
KAWAMURA Satoshi,
HIROHASHI Atsuko,
KATO Terushige,
YASUDA Mineo
Publication year - 1990
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.1990.tb00498.x
Subject(s) - staining , adipose tissue , clearance , alizarin red , fetus , skinning , skeleton (computer programming) , anatomy , chemistry , bone tissue , pathology , biology , medicine , biochemistry , pregnancy , urology , genetics
A rapid procedure was developed for staining fetal rat specimens. Full‐term rat fetuses were fixed in 99% ethanol and only thoracoabdominal organs were removed. Without skinning and removing adipose tissue and eye balls, the specimens were placed in 85% ethanol containing 1.5% KOH and 0.0015% alizarin red S for 5 days. The specimens were macerated in 1% aqueous KOH and cleared in 50% glycerin solution. The skeleton was visible through surrounding tissue containing adipose tissue which became transparent.