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Investigation of DNA Synthesis and Cell Condensation in the Developing Rat Hand Plate by the BrdU/anti‐BrdU Technique
Author(s) -
OHSHIO Itaru,
OGINO Toshihiko,
NAGASHIMA Kazuo
Publication year - 1990
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.1990.tb00492.x
Subject(s) - mesenchyme , mesenchymal stem cell , dna synthesis , dna , bromodeoxyuridine , microbiology and biotechnology , cell , biology , cell growth , chemistry , genetics
DNA synthesis and cell condensation in the developing hand plate of the rat were examined by a BrdU/anti‐BrdU immunohistochemical study. DNA‐synthesizing cells were distributed uniformly in the mesenchyme of the hands until day 12.5 in rat embryos. From day 13.0, these cells were distributed in the interdigital mesenchymal areas, leaving rare labeled cells in sites of cell condensation (i.e., in the digital rays). After day 14.5, DNA‐synthesizing cells were confined to the interdigital areas near the digital rays. On day 15.0, DNA‐synthesizing cells appeared in the areas of joint formation. In the epidermis. DNA‐synthesizing cells were uniformly found in the period of digital‐ray formation. When the mesenchymal cells labeled on day 12.0 were followed from day 12.5 until day 14.0 of embryogenesis, they were found to become concentrated in the digital rays. From these findings, we conclude that the cells which are synthesizing DNA and actively dividing in the mesenchyme at day 12.0 migrate to the digital rays, where they undergo cell condensation. The experimental models and techniques employed in this study will be useful for investigations of normal development as well as teratogenesis of the extremities.