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Biochemical and Morphological Investigation on the Ataxic Mutant Shambling Mouse
Author(s) -
YAMAGUCHI Tomoko,
KATAGIRI Noriko,
IHNUMA Mono
Publication year - 1985
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.1985.tb01005.x
Subject(s) - enolase , biochemistry , ataxia , biology , ataxic gait , chemistry , microbiology and biotechnology , neuroscience , immunohistochemistry , immunology
Ataxic Shambling mouse was investigated biochemically as well as morphologically to find any specific changes which would be compatible with their ataxic behaviors. Biochemical studies revealed that calcium uptake by the brain synaptosomes of ataxic mouse was decreased to about 30% at 30 mM potassium medium of the control and that the neuron specific enolase activity was lowered a half the value of control synaptosomes, while morphological investigations by Baker's staining for phospholipids disclosed little evidences of phospholipidosis in the nervous tissues and in other organs of the ataxic Shambling mouse. NAD dehydrogenase as well as myosin ATPase staining on the hindlimb muscles of the ataxic Shambling mouse showed a local atrophy and a certain derangement of muscle fibers. These results were discussed in relation to our previous finding of lowered phosphatidylserine synthesis by the brain microsomes which participated at the neuronal transport and transmission in the synaptosomes. Consequently, the ataxic behaviors of Shambling mouse would be ascribed to defective metabolism of phspholipids and decreased brain specific enolase in the synaptosomal fractions which may possibly result in reduced calcium uptake by the synaptosomes regulating neural transmission processes.