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Alterations in Amniotic Fluid Volume and Associated Reduction Deformities of the Limbs in Rat Induced by Cyclophosphamide
Author(s) -
SINGH Shamer,
JEYASEELAN Nadankutty
Publication year - 1985
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/j.1741-4520.1985.tb01002.x
Subject(s) - oligohydramnios , amniotic fluid , fetus , medicine , gestation , andrology , cyclophosphamide , pregnancy , endocrinology , biology , chemotherapy , genetics
Maternal administration of a single dose (20 mg/kg) of cyclophosphamide on day 12 of gestation in CF rats (Sperm += day zero) resulted in the reduction deformities of the limbs of the fetuses and increase in the volume of amniotic fluid at term. Chronological observations of 535 fetuses at 24 hour intervals from day 13 through day 20 revealed variations in the volume of amniotic fluid. On day 13 and 14 it was markedly reduced as compared to corresponding controls (P < 0.001). Though there was a gradual rise on day 15 and 16 it was again decreased during day 17 to 19. However, on day 20 it was found to be markedly increased. The associated oligohydramnios observed during the organo‐genetic period probably has a role in the causation of reduction deformities of the fetal limbs.