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Cytotoxic effects of denture adhesives on primary human oral keratinocytes, fibroblasts and permanent L 929 cell lines
Author(s) -
Chen Fengying,
Wu Tianfu,
Cheng Xiangrong
Publication year - 2014
Publication title -
gerodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 54
eISSN - 1741-2358
pISSN - 0734-0664
DOI - 10.1111/j.1741-2358.2012.00681.x
Subject(s) - cytotoxicity , mtt assay , adhesive , medicine , dentistry , agar , cytotoxic t cell , cell culture , primary culture , agar diffusion test , in vitro , chemistry , materials science , biology , biochemistry , composite material , layer (electronics) , bacteria , genetics , escherichia coli , gene
Background To date, there have been very little data on the cytotoxic responses of different cell lines to denture adhesives. Objective To determine the cytotoxicity of three denture adhesives on primary human oral keratinocytes ( HOKs ), fibroblasts ( HOFs ) and permanent mouse fibroblasts cell lines ( L 929). Methods Three commercial denture adhesives (two creams and one powder) were prepared for indirect contact using the agar diffusion test, as well as extracts in MTT assay. The results of the MTT assay were statistically analysed by one‐way anova and T ukey's test ( p < 0.05). Results All of the tested denture adhesives showed mild to moderate cytotoxicity to primary HOKs ( p < 0.001), whereas none of three was toxic to L 929 cells ( p > 0.05) in both assays. For primary HOFs cultures, slight cytotoxicity was observed for one of the products from the agar diffusion test and undiluted eluates of all tested adhesives with MTT assay ( p < 0.01). Conclusion Denture adhesives are toxic to the primary HOKs and HOFs cultures, whereas non‐toxic to L 929 cells. The results suggest that primary human oral mucosal cells may provide more valuable information in toxicity screening of denture adhesives.