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Effect of water storage and heat treatment on the cytotoxicity of soft liners
Author(s) -
Tay Lidia Yileng,
Herrera Daniel Rodrigo,
Quishida Cristiane Campos Costa,
Carlos Iracilda Zeppone,
Jorge Janaina Habib
Publication year - 2012
Publication title -
gerodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 54
eISSN - 1741-2358
pISSN - 0734-0664
DOI - 10.1111/j.1741-2358.2011.00463.x
Subject(s) - cytotoxicity , cytotoxic t cell , medicine , andrology , in vitro , toxicology , zoology , biochemistry , chemistry , biology
doi: 10.1111/j.1741‐2358.2011.00463.x Effect of water storage and heat treatment on the cytotoxicity of soft liners Objective: To evaluate the effect of water storage time on the cytotoxicity of soft liners. Methods: Sample discs of soft liners Dentusoft, Dentuflex, Trusoft, Ufi‐Gel‐P and denture base acrylic resin Lucitone‐550 were prepared and divided into four groups: GN: No treatment, G24: Stored in water at 37°C for 24 h; G48: Stored in water at 37°C for 48 h, GHW: Immersed in water at 55°C for 10 min. To analyse the cytotoxic effect, three samples of each group were placed in tubes with Dubelcco’s Modified Eagle Mediums and incubated at 37°C for 24 h. During this period, the toxic substances were leached to the culture medium. The cytotoxicity was analysed quantitatively by the incorporation of radioactivity 3 H‐thymidine checking the number of viable cells (synthesis of DNA). The data were statistically analysed using two‐way anova and Tukey’s honestly significant difference tests (α = 0.05). Results: Treatments did not reduce the cytotoxicity effect of the soft liners ( p > 0.05). It was found that Ufi‐Gel‐P had a non‐cytotoxic effect, Trusoft had a slightly cytotoxic effect, Dentuflex had a moderated cytotoxic effect, Dentusoft alternated between slightly and non‐cytotoxic effect, and Lucitone‐550 had non‐cytotoxic effect when stored in water for 48 h. Conclusion: The effect of water storage and the heat treatment did not reduce the cytotoxicity of the soft liners.