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Antimicrobial activity of disinfectant agents incorporated into type IV dental stone
Author(s) -
de Paula Pereira Rodrigo,
Lucas Matheus Guilherme,
Spolidorio Denise Madalena Palomari,
Filho João Neudenir Arioli
Publication year - 2012
Publication title -
gerodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 54
eISSN - 1741-2358
pISSN - 0734-0664
DOI - 10.1111/j.1741-2358.2011.00462.x
Subject(s) - disinfectant , antimicrobial , chlorhexidine , distilled water , candida albicans , microbiology and biotechnology , medicine , microorganism , staphylococcus aureus , agar diffusion test , food science , dentistry , bacteria , biology , chemistry , chromatography , genetics , pathology
doi: 10.1111/j.1741‐2358.2011.00462.x
Antimicrobial activity of disinfectant agents incorporated into type IV dental stone Purpose: This study evaluated the antimicrobial activity of two disinfectant agents, 2% chlorhexidine digluconate solution (CHX) and 98% chlorhexidine hydrochloride powder (HYD), incorporated into type IV dental stone at the time of mixing. Material and methods: Agar diffusion test was used for the following microorganisms: Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans . The specimens were grouped in: (1) dental stone mixed with sterile distilled water; (2) paper disc soaked with CHX; (3) dental stone mixed with CHX; and (4) dental stone with incorporation of HYD, in 1% proportion of the dental stone mass and mixed with sterile distilled water. The culture medium was inoculated with microbial suspensions 1 and 24 h after pouring of the dental stone. The antimicrobial activity was evaluated by the average diameter of microbial growth inhibition zones. The data were analysed with a nested anova ( p < 0.05) and Tukey test for specific comparisons. Results: The disinfectant agents demonstrated antimicrobial activity against all microorganisms, with the exception of C. albicans , against which the CHX was ineffective in two periods of analysis. Significant differences between disinfectants were found with all microorganisms. Conclusion: The disinfectant agents analysed were effective against most of the microorganisms tested, except C. albicans .