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Influence of Fixation on Visualization of Mitosis in Dental Pulp, and in L Cells in Vitro
Author(s) -
Rodrigues Homero H.,
Spågberg Larz,
Langeland Kaare
Publication year - 1982
Publication title -
gerodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 54
eISSN - 1741-2358
pISSN - 0734-0664
DOI - 10.1111/j.1741-2358.1982.tb00338.x
Subject(s) - mitosis , medicine , glutaraldehyde , fixation (population genetics) , pulp (tooth) , dentistry , in vitro , pathology , microbiology and biotechnology , biology , biochemistry , population , environmental health
Three tissue fixatives were evaluated for their efficacy in demonstration of mitosis in the human dental pulp and in cultured fibroblasts. Cyanuric chloride doubled the possibility of detecting mitosis in cultured cells, and increased the possibility of its detection in the pulp. Formalin and glutaraldehyde fixation were less satisfactory for the study of mitosis. With one exception, mitosis was observed only in inflamed pulps. The study indicates methodology in dental pulp studies in the geriatric patient.