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MEASUREMENT OF TUMOR HYPOXIA IN SPONTANEOUS CANINE SARCOMAS
Author(s) -
Bruehlmeier Matthias,
KaserHotz Barbara,
Achermann Roger,
Bley Carla Rohrer,
Wergin Melanie,
Schubiger Pius A.,
Ametamey Simon M.
Publication year - 2005
Publication title -
veterinary radiology and ultrasound
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.541
H-Index - 60
eISSN - 1740-8261
pISSN - 1058-8183
DOI - 10.1111/j.1740-8261.2005.00065.x
Subject(s) - medicine , fibrosarcoma , hypoxia (environmental) , perfusion , nuclear medicine , sarcoma , positron emission tomography , tumor hypoxia , osteosarcoma , pathology , oxygen , radiation therapy , chemistry , organic chemistry
We used positron emission tomography (PET) with [ 18 F]fluoromisonidazole ([ 18 F]FMISO) to study tumor hypoxia in six dogs with spontaneous sarcomas. The tumors were regarded as hypoxic if [ 18 F]FMISO uptake exceeded normal tissue radioactivity by 40% (tumor/muscle ratio >1.4) or if kinetic analysis indicated a positive [ 18 F]FMISO tissue influx rate ( K i >0) by a Patlak plot. Using these criteria, we found hypoxia in a fibrosarcoma grade II, an undifferentiated sarcoma, and an ostoeosarcoma, but not in a fibrosarcoma grade I, another osteosarcoma, and a myxosarcoma. In three animals, the tumor oxygen partial pressure ( p O 2 ) was also measured invasively using Eppendorf needle electrodes. In these cases, the Eppendorf measurements were confirmed by the [ 18 F]FMISO PET results. In addition, [ 15 O]H 2 O PET was performed in four dogs in order to assess tumor perfusion. Comparisons of the [ 18 F]FMISO with [ 15 O]H 2 O PET images in two cases showed that tumor hypoxia occurred in the tumor center with low perfusion, whereas perfusion was heterogeneous in a nonhypoxic tumor.