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EVALUATION OF SOLID PHASE RADIOLABELS OF DOG FOOD FOR GASTRIC EMPTYING
Author(s) -
Kunze Christopher P.,
Hoskinson James J.,
Butine Michael D.,
Goggin Justin M.
Publication year - 1999
Publication title -
veterinary radiology and ultrasound
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.541
H-Index - 60
eISSN - 1740-8261
pISSN - 1058-8183
DOI - 10.1111/j.1740-8261.1999.tb01904.x
Subject(s) - medicine , digestion (alchemy) , gastric emptying , chromatography , scintigraphy , stomach , food science , gastroenterology , chemistry , nuclear medicine
99m Tc‐Pertechnetate, 99m Tc‐mebrofenin, 99m Tc‐disofenin, 99m Tc‐sulfur colloid, and 99m Tc‐Dowex resin beads were evaluated for in vitro stability as a label for both dry extruded, and canned dog food for gastric emptying scintigraphy. A sample of each radiolabeled diet was added to water, gastric juice, intestinal juice, or gastric juice followed by intestinal juice for in vitro digestion. After a 3‐hour digestion period, tubes were centrifuged and percentage solid phase retention (%SPR) was calculated. The experiment was repeated three times over a 14‐day period to minimize day‐to‐day variation. For dry dog food, 99m Tc‐mebrofenin and 99m Tc‐disofenin had similar %SPR in water (96 and 93%, respectively) and gastric juice (>95% each) and were significantly higher than other labels. For canned dog food, mebrofenin had a 91% SPR for the water or gastric juice digestions, and 99m Tc‐Dowex had a %SPR of >99%. 99m Tc‐mebrofenin and 99m Tc‐Dowex were also tested in vivo, where 99m Tc‐Dowex had poor stability, and 99m Tc‐Mebrofenin had excellent stability. 99m Tc‐Mebrofenin is a suitable label for dog food for gastric emptying scintigraphy.