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IN VITRO TESTING OF 99m Tc‐LABELED MONOCLONAL ANTIBODIES SPECIFIC FOR DIROFILARIA IMMITIS
Author(s) -
Daniel Gregory B.,
Allhands Rodger V.
Publication year - 1988
Publication title -
veterinary radiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.541
H-Index - 60
eISSN - 1740-8261
pISSN - 0196-3627
DOI - 10.1111/j.1740-8261.1988.tb01742.x
Subject(s) - dirofilaria immitis , polyclonal antibodies , monoclonal antibody , sephadex , chemistry , antibody , in vitro , monoclonal , chromatography , microbiology and biotechnology , biology , biochemistry , immunology , enzyme , helminths
Monoclonal antibodies specific for Dirofilaria immitis were labeled with technetium‐99m ( 99W Tc), and their affinity was assessed in vitro. Of six monoclonal antibodies tested, three were IgG and three were IgM. A canine polyclonal IgG was used to establish the labeling technique and later as a control. Diethylenetriaminepentaacetic acid (DTPA) was used as a bifunctional chelator to bind 99m Tc covalently to the antibody. Radiochemical purity was measured with the use of gel filtration by a Sephadex G‐25 column linked to an absorbance monitor and with instant thin‐layer chromatography. The radiochemical purity had no significant influence on percent uptake on the heartworms. The clone designated as Di 130.1 had a significantly higher percent uptake than other clones tested.