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Long‐term dietary supplementation of organic selenium modulates gene expression profiles in leukocytes of adult pigs
Author(s) -
Song KiDuk,
Dowd Scott E.,
Lee HakKyo,
Kim Sung Woo
Publication year - 2013
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2012.01060.x
Subject(s) - selenium , immune system , biology , gene expression , gene , cd8 , andrology , endocrinology , medicine , immunology , microbiology and biotechnology , chemistry , biochemistry , organic chemistry
Seventy‐two pigs at 34.4 kg body weight ( BW ) were allotted to two treatments with six replicates/treatment and six pigs/pen: the CON (negative control, no added selenium ( S e)) and the OS (0.36 mg/kg added selenium from selenium‐enriched yeast). Pigs were fed until 130 kg BW . The CON diet contained 0.18 mg/kg indigenous S e whereas the OS diet contained 0.54 mg/kg S e. Blood samples were collected at 130 kg BW and further processed for microarray analysis, prepared with 885 genes related to immune function of pigs. Among those, 28 genes related to improved immune status and innate immunity were up‐regulated ( P < 0.05) in leukocytes from S e‐fed pigs and those include major histocompatibility class I (> 1.66), arginase I (> 1.27), integrin beta‐1‐subunit (> 1.20), toll like receptor 2 (> 1.12) and double‐stranded RNA ‐dependent protein kinase. However, 24 genes including tissue factor (< 4.70), serum amyloid A ‐2 protein (< 3.11) and p27 K ip1 (< 1.42) were down‐regulated ( P < 0.05) in leukocytes from S e‐fed pigs. Expression of four selected genes was validated using quantitative PCR (q PCR ) showing significant correlation between mircroarray analysis and q PCR analysis. This study indicates that a long‐ term dietary supplementation (0.3%) of organic Se improves the expression of genes that are related to enhanced immunity of pigs.