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Effects of dietary lysine levels on apparent nutrient digestibility and cationic amino acid transporter mRNA abundance in the small intestine of finishing pigs, Sus scrofa
Author(s) -
WANG XiuQi,
ZENG PeiLing,
FENG You,
ZHANG ChangMing,
YANG JingPei,
SHU Gang,
JIANG QingYan
Publication year - 2012
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2011.00941.x
Subject(s) - lysine , ileum , jejunum , biology , amino acid , nutrient , small intestine , digestion (alchemy) , messenger rna , amino acid transporter , biochemistry , zoology , chemistry , food science , transporter , chromatography , gene , ecology
One hundred and twenty pigs were used to evaluate the effects of different dietary lysine levels on the growth performance, apparent nutrient digestibility, and abundance of cationic amino acid transporter messenger RNA (mRNA) in the small intestine of finishing pigs. Pigs received a low lysine diet (LL, 0.60% lysine), moderate lysine diet (ML, 0.80% lysine) or a high lysine diet (HL, 1.00% lysine) for 28 days. A digestion test was carried out during the third week. Although the apparent nutrient digestibility in pigs fed experimental diets were different ( P < 0.05) and the highest when pigs were fed ML diet, diets did not change the growth performance. In the duodenum, mRNA abundance of PepT‐1, as detected by real‐time RT‐PCR, was reduced in the LL diet ( P < 0.05). A greater abundance of b 0,+ AT and PepT‐1 mRNA was associated with the ML diet ( P < 0.05) in the jejunum and ileum, respectively. In the ileum, the HL diet had a lower abundance of CAT‐1 mRNA compared with other diets. These results showed that the finishing pigs would gain better nutrient digestibility when the dietary lysine content was 0.80%, and dietary lysine levels influenced the expression of cationic amino acid transporter mRNA in the small intestine of finishing pigs.