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Contribution of blastoderm cells to Japanese quail ( Coturnix coturnix japonica )–Peking duck ( Anas platyrhynchos ) chimeras
Author(s) -
GAO Junshuang,
YUAN Fang,
TANG Xiaoyan,
HAN Haitang,
SHA Jin,
YUAN Jihong,
SHAO Yujing,
JIN Xiumei,
LIU Haiwen,
RUI Lei,
LI Zandong
Publication year - 2011
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2011.00905.x
Subject(s) - blastoderm , quail , biology , coturnix coturnix , chimera (genetics) , embryo , hatching , andrology , anatomy , microbiology and biotechnology , embryogenesis , genetics , zoology , endocrinology , gene , medicine
The purpose of this study was to produce quail–duck chimeras by transferring stage X blastoderm cells and to detect the distribution of donor cells in heterogeneous embryos using PCR. Four experimental groups were made by transferring different amounts of quail blastoderm cells into duck recipients. In early embryonic stages, donor cells labeled with PKH26 fluorescent dye were observed in the head, neural tube and gonads by fluorescent microscopy. A total of 194 duck recipient embryos were injected and 93 survived to hatch. The average hatching rate was 48% (93/194); the hatching rate showed a significant difference among all the groups ( P < 0.05). Sixteen somatic chimeras were obtained, 10 of which had black feathers derived from the donor quail. The PCR results showed that donor cells were distributed in various tissues and organs of the phenotypic chimeras. This is the first report on producing Japanese quail–Peking duck chimeras by transferring quail blastoderm cells into the subgerminal cavity of the duck. This technique will provide a basis for the investigation of fertilization barriers in interspecies germline chimeras and will aid conservation of endangered wild birds.