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The investigation of probiotic potential of lactic acid bacteria isolated from traditional Mongolian dairy products
Author(s) -
TAKEDA Shiro,
YAMASAKI Keiko,
TAKESHITA Masahiko,
KIKUCHI Yukiharu,
TSENDAYUSH Chuluunbat,
DASHNYAM Bumbein,
AHHMED Abdulatef M.,
KAWAHARA Satoshi,
MUGURUMA Michio
Publication year - 2011
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2011.00874.x
Subject(s) - probiotic , lactococcus lactis , biology , lactic acid , 16s ribosomal rna , lactobacillus paracasei , bacteria , food science , lactobacillus , microbiology and biotechnology , fermented milk products , fermentation , subspecies , lactococcus , genetics , paleontology
ABSTRACT The aims of this study were to investigate the diversity of lactic acid bacteria (LAB) isolated from traditional Mongolian dairy products, and to estimate the probiotic potential of the isolated strains. We collected 66 samples of the traditional Mongolian dairy products tarag ( n = 45), airag ( n = 7), aaruul ( n = 8), byasulag ( n = 1) and eezgii ( n = 5), from which 543 LAB strains were isolated and identified based on 16S ribosomal DNA sequence. The predominant species of those products were Lactobacillus ( L .) delbrueckii ssp. bulgaricus , L . helveticus , L . fermentum , L . delbrueckii ssp. lactis and Lactococcus lactis ssp. lactis . However, we could not detect any LAB strains from eezgii. All LAB isolates were screened for tolerance to low pH and to bile acid, gas production from glucose, and adherence to Caco‐2 cells. In vitro , we found 10 strains possess probiotic properties, and almost identified them as L. plantarum or L. paracasei subspecies, based on 16S ribosomal DNA and carbohydrate fermentation pattern. These strains were differentiated from each other individually by randomly amplified polymorphic DNA analysis. Additionally, it was notable that 6/10 strains were isolated from camel milk tarag from the Dornogovi province.