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Enhancement of viability of a probiotic Lactobacillus strain for poultry during freeze‐drying and storage using the response surface methodology
Author(s) -
KHORAMNIA Anahita,
ABDULLAH Norhani,
LIEW Siew Ling,
SIEO Chin Chin,
RAMASAMY Kalavathy,
HO Yin Wan
Publication year - 2011
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2010.00804.x
Subject(s) - probiotic , food science , strain (injury) , lactobacillus , microbiology and biotechnology , poultry meat , response surface methodology , poultry farming , food storage , freeze drying , chemistry , biology , bacteria , veterinary medicine , chromatography , medicine , fermentation , genetics , anatomy
A rotatable central composite design (CCD) was used to study the effect of cryoprotectants (skim milk, sucrose and lactose) on the survival rate of a probiotic Lactobacillus strain, L. reuteri C10, for poultry, during freeze‐drying and storage. Using response surface methodology, a quadratic polynomial equation was obtained for response value by multiple regression analyses: Y = 8.59546 − 0.01038 X 1 − 0.09382 X 2 − 0.07771 X 3 − 0.054861 X 1 2 − 0.04603X 3 2 − 0.10938 X 1 X 2 . Based on the model predicted, sucrose exerted the strongest effect on the survival rate. At various combinations of cryoprotectants, the viability loss of the cells after freeze‐drying was reduced from 1.65 log colony forming units (CFU)/mL to 0.26–0.66 log CFU/mL. The estimated optimum combination for enhancing the survival rate of L. reuteri C10 was 19.5% skim milk, 1% sucrose and 9% lactose. Verification experiments confirmed the validity of the predicted model. The storage life of freeze‐dried L. reuteri C10 was markedly improved when cryoprotectants were used. At optimum combination of the cryoprotectants, the survival rates of freeze‐dried L. reuteri C10 stored at 4°C and 30°C for 6 months were 96.4% and 73.8%, respectively. Total viability loss of cells which were not protected by cryoprotectants occurred after 12 and 8 weeks of storage at 4°C and 30°C, respectively.