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Modulatory effects of two levels of dietary Allium s on immune response and certain immunological variables, following immunization, in White Leghorn chickens
Author(s) -
HANIEH Hamza,
NARABARA Kiyoaki,
PIAO Mingzi,
GERILE Chaogetu,
ABE Asaki,
KONDO Yasuhiro
Publication year - 2010
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2010.00798.x
Subject(s) - biology , allium sativum , immune system , spleen , white blood cell , allium , lymphocyte , splenocyte , newcastle disease , cd8 , immunology , andrology , virus , medicine , botany
This study aimed at investigating the effects of dietary Allium sativum (garlic, G) and Allium cepa (onion, O) on immune functions in White Leghorn chicken. One‐week‐old chicks, were fed diets without (control) or with Allium s (GL and OL, 10 g or GH and OH, 30 g/kg diet). Chickens were immunized with Newcastle disease virus (NDV), sheep red blood cells (SRBC) and Brucella abortus (BA). Antibodies, lymphocyte proliferation, and ratios of CD4 + , CD8 + and CD4 ‐ CD8 ‐ lymphocytes were investigated. Histology and weights of the spleen, thymus and bursa (BF), and white blood cell (WBC) counts were studied as well. Allium s at 10 g/kg diet enhanced anti‐NDV, anti‐SRBC and anti‐BA antibody productions, whereas 30 g/kg diet had less stimulatory effects. Histology of the lymphoid organs and proliferation of peripheral blood lymphocytes (PBL) were not influenced. However, splenocyte and thymocyte proliferations were augmented with garlic. Flow cytometry analysis showed reduction in CD4 + and increase in CD4 ‐ CD8 ‐ lymphocyte ratios in GH and OH groups. Garlic‐supplemented chickens had heavier spleen and thymus, and higher WBC counts, whereas BF weight increased with both Alliums at 30 g/kg diet. These results suggest that dietary Allium s have a potential to enhance the immune functions in White Leghorn chickens.