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Isolation and characterisation of crocosin, an antibacterial compound from crocodile ( Crocodylus siamensis ) plasma
Author(s) -
PREECHARRAM Sutthidech,
JEARRANAIPREPAME Pornpimol,
DADUANG Sakda,
TEMSIRIPONG Yosapong,
SOMDEE Theerasak,
FUKAMIZO Tamo,
SVASTI Jisnuson,
ARAKI Tomohiro,
THAMMASIRIRAK Sompong
Publication year - 2010
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2010.00752.x
Subject(s) - crocodile , antibacterial activity , crocodylus , pronase , staphylococcus aureus , chemistry , biology , microbiology and biotechnology , bacteria , chromatography , biochemistry , ecology , genetics , trypsin , enzyme
An antibacterial compound from crocodile blood was partially purified and functionally characterised. The freshwater crocodile ( Crocodylus siamensis ) plasma with antibacterial activity was partially purified by using a centrifugal concentrator and reverse phase high powered liquid chromatography, and designated as crocosin. Crocosin exhibits antibacterial activity toward Salmonella typhi and Staphylococcus aureus . Crocosin is thermostable and resistant to pronase digestion. The structure of crocosin analyzed by mass spectrometry contains repeating units of 94 and 136 m/z. Scanning electron microscopy indicates that crocosin probably penetrates progressively into cytoplasm space, perturbing and damaging bacterial membranes. Crocosin may provide an early defense mechanism toward bacterial infection in freshwater.