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In vivo immunomodulatory effects of dietary purple sweet potato after immunization in chicken
Author(s) -
HANIEH Hamza,
GERILE Chaogetu,
NARABARA Kiyoaki,
GU Zhigang,
ABE Asaki,
KONDO Yasuhiro
Publication year - 2010
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2009.00715.x
Subject(s) - immune system , splenocyte , biology , antibody titer , cd8 , spleen , immunization , immunity , newcastle disease , eimeria , immunology , titer , cellular immunity , antibody , microbiology and biotechnology , virus
This study was intended to determine the modulatory effects of dietary supplementation of purple sweet potato ( Ipomoea batats Poir., PSP) on the immune response of chickens. PSP was included in a basal starter diet by 1% (PSP L ) or 3% (PSP H ) and continually fed. Newcastle disease (NDV) vaccine, Brucella abortus (BA) and sheep red blood cells (SRBC) were used for chicken immunization. Antibody titers against these antigens were used to estimate humoral immunity. Concanavalin A (Con A)‐induced proliferations of splenocytes, thymocytes and peripheral blood lymphocytes (PBL), ratios of CD4‐ and CD8‐single positive and CD4‐CD8‐double negative (DN) cells in splenocytes, were both used to indicate cellular immunity. Relative weights of spleen, thymus and bursa and white blood cell (WBC) counts were studied. PSP H increased anti‐NDV ( P < 0.05), anti‐BA ( P < 0.01) and anti‐SRBC titers ( P < 0.05) in response to secondary immunization, whereas PSP L increased titers of anti‐BA ( P < 0.05) and anti‐SRBC ( P < 0.01). Proliferations of splenocytes and thymocytes were augmented with PSP L ( P < 0.05). PSP H ‐treated chickens had lower ( P < 0.05) ratios of CD4‐sigle positive lymphocytes. Proliferation of PBL, weights of lymphoid organs and WBC counts were not affected. These results suggest that dietary PSP supplementation could enhance the immune response after immunization in chickens.