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Identification of a potent immunostimulatory oligodeoxynucleotide from Streptococcus thermophilus lac Z
Author(s) -
SHIMOSATO Takeshi,
TOHNO Masanori,
SATO Takashi,
NISHIMURA Junko,
KAWAI Yasushi,
SAITO Tadao,
KITAZAWA Haruki
Publication year - 2009
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2009.00680.x
Subject(s) - biology , cd86 , tlr9 , cd80 , cpg oligodeoxynucleotide , microbiology and biotechnology , immune system , cd11c , innate immune system , t cell , gene expression , cd40 , in vitro , immunology , gene , biochemistry , phenotype , dna methylation , cytotoxic t cell
Immunostimulatory sequences of oligodeoxynucleotides (ODNs), such as CpG ODNs, are potent stimulators of innate immunity. Here, we identified a strong immunostimulatory CpG ODN, which we named MsST, from the lac Z gene of Streptococcus (S.) thermophilus ATCC19258, and we evaluated its immune functions. In in vitro studies, MsST had a similar ability as the murine prototype CpG ODN 1555 to induce inflammatory cytokine production and cell proliferation. In mouse splenocytes, MsST increased the number of CD80+CD11c+and CD86+CD11c+ dendritic cells and CD4+CD25+ regulatory T cells. We also analyzed the effects of MsST on the expression of regulatory cytokines by real‐time quantitative PCR. MsST was more potent at inducing interleukin‐10 expression than the ODN control 1612, indicating that MsST can augment the regulatory T cell response via Toll‐like receptor 9, which plays an important role in suppressing T helper type 2 responses. These results suggest that S. thermophilus , whose genes include a strong Immunostimulatory sequence‐ODN, is a good candidate for a starter culture to develop new physiologically functional foods and feeds.