Premium
B cell differentiation in the bursa of Fabricius and spleen of embryos and chicks immediately after hatching
Author(s) -
NARABARA Kiyoaki,
ABE Asaki,
HANIEH Hamza,
KONDO Yasuhiro
Publication year - 2009
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2009.00679.x
Subject(s) - bursa of fabricius , spleen , hatching , biology , embryo , andrology , population , splenocyte , antibody , embryogenesis , immunology , microbiology and biotechnology , zoology , medicine , environmental health
Flow cytometric analysis and immunohistochemical observation were used to qualitatively and quantitatively clarify the nature of B cell differentiation in the bursa of Fabricius of chick embryos and to determine the timing of antibody class switching in chicken spleens based on positivity of IgM and IgG on and in the cells. In the bursa, the sIgM‐positive cell population formed from the 12 th to 15 th day of embryogenesis. The proportion of sIgM‐high expressing (sIgM high ) cells was lower among bursacytes than splenocytes of hatched chicks, suggesting that the sIgM high bursacytes are to be released to peripheral sites. The proportion of sIgM high cells was higher at 0 days old than at any other examined stage of development. Colonization of the spleen by B cells occurred between the 18 th day of embryogenesis and 0 days old. Antibody class switching was thought to start in the spleen between 1 and 2 weeks of age, because IgG‐positive cells were present in the spleen of 2‐week‐old chicks, but not 0‐day‐old or 1‐week‐old chicks.