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Methodology for the study of the hypothalamic‐pituitary hormone secretion in cattle
Author(s) -
HASHIZUME Tsutomu,
KASUYA Etsuko
Publication year - 2009
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2008.00589.x
Subject(s) - microdialysis , hormone , anterior pituitary , secretion , medicine , endocrinology , hypothalamic hormones , in vivo , pituitary hormones , in vitro , biology , perfusion , hypothalamus , central nervous system , biochemistry , microbiology and biotechnology
Studies on the neuroregulatory mechanisms on the secretion of anterior pituitary (AP) hormones in domestic animals are important because nearly all complex physiological and metabolic processes are regulated by the AP hormones. To examine them, this article considers in vivo approaches such as the techniques of intrahypothalamic injection, intracerebroventricular injection, push‐pull perfusion, and microdialysis, which have been employed in our own research group for the study in cattle. Also, in vitro approaches such as bovine AP cell culture and the AP explants superfusion system are described. This article clarifies the potential of neuroendocrine study techniques in cattle.