Premium
Cryopreservation of Black Bengal buck semen: Effects of diluents and freezing on sperm motility and morphology
Author(s) -
AFROZ Sadia,
ISLAM Md. Rashedul,
KHANDOKER M.A.M. Yahia,
AKTER Quzi Sharmin
Publication year - 2008
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2008.00563.x
Subject(s) - semen , diluent , sperm , extender , cryopreservation , sperm motility , andrology , semen cryopreservation , motility , semen extender , artificial insemination , biology , tris , chemistry , insemination , anatomy , biochemistry , medicine , nuclear chemistry , genetics , embryo , pregnancy , organic chemistry , polyurethane
Semen from Black Bengal bucks was collected to establish a cooling protocol (to −196°C) for buck semen preservation, and to study the effect of freezing on sperm motility and morphology. Semen was diluted with diluents (Triladyl & Tris) and cryoprotectants, filled into straws, sealed, cooled (to 5°C) and equilibrated. After dilution, motility ranged from 75.00% to 76.67% and from 73.33% to 80.00% in Triladyl and Tris diluents, respectively. Motility of sperm after cooling to 5°C in Triladyl and Tris diluents ranged from 65.00% to 66.67% and from 63.33% to 70.00%, respectively. After equilibration in straws, the semen was subjected to a freezing protocol in a computer‐controlled biofreezer CL‐3000 (cooling at 10°C per minute, from 5°C to −80°C) and plunged into liquid nitrogen. Sperm motility of re‐thawed semen varied from 38.33% to 43.33% and from 6.00% to−6.67% in Triladyl and Tris diluents, respectively. Sperm morphology of re‐thawed semen was studied and head damage or cryoinjury was found in 2–3% of sperm in Triladyl diluents and 3–6% in Tris diluents. Whether the differences of sperm motility and head damage reflect fertility after artificial insemination is yet unknown and needs to be studied further.