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Age‐related changes in leukocytes and T cell subsets in peripheral blood of Japanese Black cattle
Author(s) -
TANAKA Sachi,
MIYAZAWA Kohtaro,
KUWANO Atsuko,
WATANABE Kouichi,
OHWADA Shyuichi,
ASO Hisashi,
NISHIDA Shigeru,
YAMAGUCHI Takahiro
Publication year - 2008
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2008.00539.x
Subject(s) - cd8 , flow cytometry , peripheral blood , biology , immune system , t cell , immunology , cell , peripheral blood mononuclear cell , monoclonal antibody , andrology , antibody , medicine , genetics , in vitro
It is well known that the immune system changes with age during development and maturation in Holstein cattle. But age‐related changes in leukocytes and T cell subsets in peripheral blood of Japanese Black cattle still remain unclear. The aim of the present study was to investigate comparative changes of leukocytes (granulocytes, monocytes, B cells and T cells) and T cell subsets (CD4 + , CD8 + , γδ, CD8 + γδ and WC1 + γδ T cells) in Japanese Black cattle aged 0.5, 1, 2, 6, 18 and 36–41 (adult) months on flow cytometry using specific monoclonal antibodies for the cell surface markers. T cell proportion was approximately 40% in 2‐month‐old cattle and decreased to 20.6% in adults. In contrast, B cell proportion significantly increased from 7.4% to 28.2% with age. In T cell subsets the percentage of CD4 + T cells significantly increased from 40.5% to 60%, but that of WC1 + γδ T cell subset significantly decreased with age. The percentages of CD8 + and CD8 + γδ T cells did not change. The present study details the proportional changes in leukocyte and T cell subsets with age in the peripheral blood of Japanese Black cattle and these findings are similar to those described for Holstein cattle.