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Three‐dimensional reconstruction of intramuscular collagen networks of bovine muscle: A demonstration by an immunohistochemical/confocal laser‐scanning microscopic method
Author(s) -
NAKAMURA YoshiNori,
IWAMOTO Hisao,
YAMAGUCHI Takahiro,
ONO Yoshitaka,
NAKANISHI Yoshitaka,
TABATA Shoji,
NISHIMURA Shotaro,
GOTOH Takafumi
Publication year - 2007
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2007.00460.x
Subject(s) - endomysium , confocal , immunohistochemistry , confocal microscopy , anatomy , confocal laser scanning microscopy , chemistry , laser scanning , pathology , laser , biophysics , biology , microbiology and biotechnology , medicine , optics , physics , coeliac disease , disease
We undertook a three‐dimensional reconstruction of intramuscular collagen networks of bovine muscle using an immunohistochemical/confocal laser‐scanning microscopic method. By immunohistochemical staining, type I and III collagens were observed mainly in the perimysium, while type IV collagen was observed in the endomysium. On the other hand, type V and VI collagens were observed in both the perimysium and endomysium. By confocal laser‐scanning microscopy, the collagen observed in the perimysium was three‐dimensionally reconstructed as plate‐shaped layers whereas the collagen observed in the endomysium surrounded myofibers. The three‐dimensionally reconstructed observations using immunohistochemical/confocal laser‐scanning microscopic method is useful for investigating collagen networks in muscle.