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Application of time‐resolved fluorometry to immunoassays for bovine reproductive hormones
Author(s) -
KANEKO Hiroyuki,
HASEGAWA Yoshihisa
Publication year - 2007
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2006.00398.x
Subject(s) - hormone , radioimmunoassay , immunoassay , fluorescence spectroscopy , chemistry , regulator , follicle stimulating hormone , endocrinology , medicine , biology , chromatography , fluorescence , biochemistry , immunology , antibody , luteinizing hormone , physics , quantum mechanics , gene
The principle of time‐resolved fluorometry with lanthanide chelates was established in the 1980s, but in the field of animal sciences it has not been widely applied to immunoassays. However, immunoassays that utilize time‐resolved fluorometry are possible alternatives to radioimmunoassays, since they can attain high sensitivity without safety risks. In this short review, we introduce the development of time‐resolved immunoassays for inhibin A, inhibin B and follicle‐stimulating hormone (FSH), and describe their application to the investigation of FSH regulation in male and female cattle. The results obtained using these newly developed immunoassays indicate that inhibin A acts as a feedback regulator for FSH secretion in female cattle, whereas inhibin A, and probably inhibin B, do so in male cattle.