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Effect of propionate, pyruvate and β‐hydroxybutyric acid on pyruvate carboxylase mRNA expression of in vitro culture bovine hepatocytes
Author(s) -
XU Chuang,
WANG Zhe
Publication year - 2006
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2006.00370.x
Subject(s) - pyruvate carboxylase , propionate , gluconeogenesis , pyruvate decarboxylation , pyruvate dehydrogenase kinase , pkm2 , biochemistry , pyruvate dehydrogenase phosphatase , chemistry , biology , endocrinology , medicine , microbiology and biotechnology , metabolism , pyruvate kinase , enzyme , glycolysis
The objective was to detect the effect of propionate, pyruvate and β‐hydroxybutyric acid on pyruvate carboxylase mRNA expression in in vitro culture bovine hepatocytes through a quantity reverse transcription polymerase chain reaction method. Pyruvate carboxylase is a key enzyme in the liver gluconeogenesis of ruminants and plays an important role in the negative energy balance of peri‐parturient dairy cows. The effect of propionate, pyruvate and β‐hydroxybutyric acid on pyruvate carboxylase mRNA expression at cell level is presented in this article. In experiment 1, the various concentrations of propionate from 0 mmol/L to 11.5 mmol/L, were added to the hepatocytes media. The results indicate that the level of pyruvate carboxylase mRNA was significantly enhanced accompanying increased propionate concentrations in the media ( P < 0.01) and high concentrations of propionate did not inhibit the levels of pyruvate carboxylase mRNA. In experiment 2, pyruvate in concentrations from 0 mmol/L to 12 mmol/L was added to the media. The results indicate that the levels of pyruvate carboxylase mRNA were significantly enhanced when concentrations of pyruvate increased from 0 mmol/M to 4 mmol/M and decreased when concentrations of pyruvate increased from 8 mmol/M to 12 mmol/M ( P < 0.01). In experiment 3, β‐hydroxybutyric acid of various concentrations from 0 mmol/L to 3.0 mmol/L was added to the media. The results indicate that pyruvate carboxylase mRNA levels increased slightly when concentrations of β‐hydroxybutyric acid were less than 1 mmol/L, and pyruvate carboxylase mRNA levels decreased significantly when they exceeded 2 mmol/L or 3 mmol/L ( P < 0.01). There results suggest that β‐hydroxybutyric acid inhibits PC mRNA expression.