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Determination of nucleotide sequence of SRY gene in sika deer ( Cervus nippon )
Author(s) -
FUKUI Emiko,
KOGANEZAWA Masaaki,
YOSHIZAWA Midori
Publication year - 2006
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2006.00345.x
Subject(s) - testis determining factor , cervus , biology , open reading frame , genetics , nucleic acid sequence , gene , phylogenetic tree , polymerase chain reaction , microbiology and biotechnology , y chromosome , peptide sequence , zoology
The aim of the present study was to determine the whole nucleotide sequence of the open reading frame of the sex‐determining region Y ( SRY‐ ORF) in wild sika deer. The SRY gene of wild sika deer was obtained by polymerase chain reaction (PCR) with DNA from blood samples. The whole nucleotide sequence of the SRY ‐ORF in wild sika deer consisted of 687 bp and encoded 229 deduced amino acids. In comparison with the bovine SRY gene , the percentage of nucleotide sequence homology was 91.0% in the overall ORF, and those of the N‐terminal, high mobility group (HMG) box, and C‐terminal regions within ORF were 88.9%, 96.2% and 87.9%, respectively. The nucleotide sequences of sika deer SRY ‐ORF characterized in the present study can be used for phylogenetic analysis or sexing in wild sika deer.