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Inhibition of growth hormone secretagogue receptor antagonist, [D‐Lys‐3]‐GHRP‐6, in adipogenesis of ovine and rat adipocytes
Author(s) -
ROH Sanggun,
HONG Yeonhee,
HISHIKAWA Daisuke,
TSUZUKI Hiroaki,
MIYAHARA Hisae,
NISHIMURA Yukihiko,
GOTO Chizu,
SUZUKI Yasuki,
CHOI Kichoon,
LEE Honggu,
SASAKI Shinichi
Publication year - 2005
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2005.00279.x
Subject(s) - adipogenesis , ghrelin , medicine , endocrinology , growth hormone secretagogue receptor , adipose tissue , adipocyte , chemistry , secretagogue , receptor , downregulation and upregulation , antagonist , biology , biochemistry , gene
Ghrelin and growth hormone secretagogues receptor (GHS‐R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS‐R in adipocytes, the effect of the GHS‐R antagonist, [D‐Lys‐3]‐GHRP‐6 (His‐D‐Trp‐D‐Lys‐Trp‐D‐Phe‐Lys‐NH 2 ), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D‐Lys‐3]‐GHRP‐6 (10 −7 mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)‐γ2 mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D‐Lys‐3]‐GHRP‐6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol‐3‐phosphate dehydrogenase and upregulation of PPAR‐γ2. Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D‐Lys‐3]‐GHRP‐6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS‐R in adipogenesis can be blocked by treatment with [D‐Lys‐3]‐GHRP‐6.