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Evidence for estrogen receptor expression in the bursal epithelial cells of chicks
Author(s) -
SHIN YoungHa,
TAKAGI Fumiko,
SUGITA Sachi,
HAN Song,
TSUJI Takehito,
ABE Asaki,
KONDO Yasuhiro
Publication year - 2005
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2005.00264.x
Subject(s) - oviduct , bursa of fabricius , biology , complementary dna , estrogen receptor , microbiology and biotechnology , primer (cosmetics) , estrogen , andrology , gene , endocrinology , virology , virus , chemistry , genetics , medicine , organic chemistry , cancer , breast cancer
The aim of the present study was to demonstrate estrogen receptor (ER) α (ER‐α) and ER‐β expression in the bursa of Fabricius and cultured bursal epithelial cells (CBEC) by reverse transcription (RT)‐polymerase chain reaction (PCR) using a primer set design based on the sequences of chicken ER‐α complementary DNA (cDNA) and chicken ER‐β‐cDNA. In the case of ER‐α, RT‐PCR products of the expected size (698 base pairs (bp)) were amplified from cDNA derived from the bursa and CBEC, and from the oviduct, which was used as a positive control. All of the products were cleaved into two fragments of 476 bp and 222 bp by treating the products with restriction nuclease Hind III, indicating that these RT‐PCR products from the bursa, CBEC and genital organs were the same. In contrast, in the case of ER‐β, PCR products of the expected size (303 bp) were not detected in the bursa of Fabricius or CBEC, or in the oviduct and ovary. These results indicate that ER‐α is expressed in the bursa of Fabricius and in CBEC, but ER‐β is not expressed in the bursa or CBEC, or in the female genital organs of chickens.