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Attempt to produce nuclear transferred primordial germ cells using electrofusion in domestic chicken
Author(s) -
MINEMATSU Takeo,
TAJIMA Atsushi,
KANAI Yukio
Publication year - 2004
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2004.00186.x
Subject(s) - electrofusion , somatic cell , somatic cell nuclear transfer , germ , embryo , biology , microbiology and biotechnology , embryonic stem cell , chemistry , andrology , embryogenesis , genetics , gene , materials science , medicine , blastocyst , metallurgy
As a step to develop a somatic nuclear transfer technique for avian species, an attempt to produce somatic nuclear transferred primordial germ cells (PGC) in the domestic chicken was carried out. Primordial germ cells and embryonic blood cells (EBC) were collected from 2‐day‐old embryos and the nuclei were transferred from EBC into PGC by electrofusion. The most efficient pearl chain was developed when a 350‐V/cm AC field was applied for 60 s. Cell fusion between PGC and EBC was most effective when 4‐kV/cm DC pulses, 60 µs pulse width, were applied three times to a cell suspension dispersed in 0.2 or 0.25 mol/L saccharose solution. The present results provide basic information for the production of somatic cell nuclear transferred chickens using PGC as the nuclear recipient.