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Dialysis culture for determination of MIC and MBC of benzylpenicillin against Borrelia burgdorferi
Author(s) -
STIERNSTEDT SUSANNE HAMMERS,
TADESSE TEKLEWEINI,
WRETLIND BENGT
Publication year - 1999
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1999.tb01568.x
Subject(s) - minimum bactericidal concentration , benzylpenicillin , minimum inhibitory concentration , borrelia burgdorferi , microbiology and biotechnology , antibiotics , incubation period , incubation , broth microdilution , chemistry , penicillin , chromatography , biology , immunology , biochemistry , antibody
Minimal inhibitory concentration (MIC) of benzylpenicillin (Pen G) against strains of Borrelia burgdorferi has earlier been determined by a recently developed dialysis culture technique which provides a constant concentration of Pen G. To further investigate this method, both MIC and minimal bactericidal concentration (MBC) of Pen G against B. burgdorferi were determined and the results were compared with those obtained by other authors using different methods. The incubation period was 7 days and subcultures for MBC were observed for a further 2 weeks. The study showed results of MIC and MBC similar to those of broth microdilution, indicating sensitivity, but lower values than the majority of MIC and MBC results reported by other authors using broth macrodilution. It is essential for the results of antibiotic sensitivity testing in vitro against slowly growing bacteria like Borrelia burgdorferi that the concentration of antibiotics such as Pen G, which are unstable in solution, is constant during the incubation period.