Premium
Tyrosinase gene expression in clear cell sarcoma indicates a melanocyte origin: insight from the first reported canine case
Author(s) -
Cangul I. Taci,
Garderen Evert,
Poel Hein J. A.,
Weijer Kees,
Misdorp Wim
Publication year - 1999
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1999.tb01500.x
Subject(s) - clear cell sarcoma , melanosome , melanoma , tyrosinase , melanocyte , biology , sarcoma , pathology , gene expression , immunohistochemistry , gene , microphthalmia associated transcription factor , reverse transcription polymerase chain reaction , polymerase chain reaction , cell , cancer research , microbiology and biotechnology , melanin , medicine , immunology , enzyme , genetics , biochemistry
The aim of this study was to characterize a metastasizing soft tissue tumor in a dog, which clinically, grossly and histologically showed a close resemblance to human clear cell sarcoma, a soft tissue variant of malignant melanoma. Ultrastructurally, melanosomes were found, indicating a melanocytic origin of the tumor. Using reverse‐transcription polymerase chain reaction, expression of the gene encoding tyrosinase was determined in tumor cells. With this first case of canine clear cell sarcoma, as well as the earlier report from our laboratory on amelanotic melanomas in the cat, we demonstrate that expression of the tyrosinase gene may occur in a broader range of less differentiated melanocytic tumors in different species, including man.