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Expression of fibroblast growth factor‐2 transcripts in the healing of acetic acid‐induced gastric ulcers
Author(s) -
Kimura Tsuguhiro,
Noda Masao,
Sugihara Hiroyuki,
Kashima Kei,
Hattori Takanori
Publication year - 1999
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1999.tb01471.x
Subject(s) - fibroblast growth factor , in situ hybridization , wound healing , fibroblast , myofibroblast , mesenchymal stem cell , angiogenesis , peripheral blood mononuclear cell , pathology , cancer research , biology , microbiology and biotechnology , messenger rna , medicine , cell culture , immunology , receptor , biochemistry , in vitro , gene , genetics , fibrosis
Basic fibroblast growth factor‐2 (FGF‐2) has an important role in angiogenesis, and has been demonstrated to promote ulcer healing. However, the actual part played by FGF‐2 in the process of ulcer healing is not well understood. In this study, we investigated expression of FGF‐2 transcripts at each stage of gastric ulcer healing, using an acetic acid model in rats. We made ulcers in the rat stomach by direct application of acetic acid, and 3 days and 1, 2, 3, 4, 8 weeks after treatment, we examined expression of FGF‐2 transcripts by in situ hybridization. On day 3, FGF‐2 transcripts were detected in mononuclear cells infiltrating the submucosal layer around the ulcer. After 1 and 2 weeks, expression of FGF‐2 transcripts was prominent in spindle‐shaped mesenchymal cells and endothelial cells, which proliferated in the ulcerative region. Some of the spindle‐shaped cells which expressed FGF‐2 transcripts also showed immunoreactivity for α‐smooth muscle actin. After 3 and 4 weeks, FGF‐2 expression was seen mainly in endothelial cells of vessels. These results suggest that different cells produce FGF‐2 during the process of gastric ulcer healing, and some of the spindle‐shaped cells expressing FGF‐2 transcripts in the early phase are myofibroblasts.