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Evaluation of an immunohistochemical test with polyclonal antibodies raised against mycobacteria used in formalin‐fixed tissue compared with mycobacterial specific culture
Author(s) -
CARABIAS E.,
PALENQUE E.,
SERRANO R.,
AGUADO J. M.,
BALLESTÍN C.
Publication year - 1998
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1998.tb01361.x
Subject(s) - polyclonal antibodies , immunohistochemistry , antiserum , pathology , h&e stain , staining , granuloma , biology , predictive value , antibody , microbiology and biotechnology , medicine , immunology
An immunohistochemical (IH) test (commercially available polyclonal antiserum rabbit anti‐ Mycobacterium bovis; DAKO A/S) was used to detect the presence of mycobacteria in 65 formalin‐fixed, paraffin‐embedded tissue blocks from different organs, showing necrotizing caseous granuloma lesions on hematoxylin and eosin sections from 65 patients. These 65 samples were dyed using an acid‐fast fluorescent technique and compared using the immunohistochemical method. Both results were also compared with the mycobacterial cultures. The IH test, compared with the culture, showed a sensitivity (S) of 52%, a specificity (Sp) of 76%, a positive predictive value (PV pos) of 61% and a negative predictive value (PV neg) of 69%. We analyze these data and discuss the possible causes of false‐positive and ‐negative results of the IH test. This rapid test on paraffin embedded tissue seems valuable in the period when waiting for the culture results.