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Towards the isolation of a human malignant extragonadal germ cell tumour‐associated breakpoint in chromosome 11q13
Author(s) -
SINKE R. J.,
ASSELDONK M.,
BRUIJN D.,
STRIJK J. A.,
MERKX G.,
WEGHUIS D. OLDE,
JONG B.,
OOSTERHUIS J. W.,
KESSEL A. GEURTS
Publication year - 1998
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1998.tb01321.x
Subject(s) - breakpoint , contig , biology , chromosomal translocation , genetics , germ cell , fluorescence in situ hybridization , comparative genomic hybridization , chromosome , cytogenetics , microbiology and biotechnology , bacterial artificial chromosome , gene , genome
In a previous study we have defined a subgroup of human malignant extragonadal germ cell tumours that is characterized by complex translocations involving chromosomes 6 and 11 ( Echten et al. 1995). Here we report (i) the use of fluorescent in situ hybridization, pulsed field gel electrophoresis and direct visual hybridization techniques to localize the tumour‐associated breakpoint within band 11q13, and (ii) the construction of a phage library enriched for this region to facilitate genomic walks towards the breakpoint. Extensive breakpoint‐flanking contigs were generated and within these contigs six candidate genes could be identified.