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Chronic Pseudomonas aeruginosa lung infection is more severe in Th 2 responding BALB/c mice compared to Th 1 responding C 3 H/HeN mice
Author(s) -
MOSER CLAUS,
JOHANSEN HELLE KROGH,
SONG ZHIJUN,
HOUGEN HANS PETTER,
RYGAARD JØRGEN,
HØIBY NIELS
Publication year - 1997
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1997.tb05092.x
Subject(s) - pseudomonas aeruginosa , balb/c , microbiology and biotechnology , lung , lung infection , immunology , medicine , biology , bacteria , immune system , genetics
The chronic Pseudomonas aeruginosa lung infection in cystic fibrosis (CF) is characterized by a pronounced antibody response and microcolonies surrounded by numerous polymorphonuclear neutrophils (PMN). Poor prognosis is correlated with a high antibody response to P. aeruginosa antigens. An animal model of this infection was established in two strains of mice: C3H/HeN and BALB/c, generally known as Th 1 and Th 2 responders, respectively, which were challenged with alginate‐embedded P. aeruginosa. Mortality was significantly lower in C3H/HeN compared to BALB/c mice (p<0.025). P. aeruginosa was cleared more efficiently in C3H/HeN mice and significantly more C3H/HeN mice showed normal lung histopathology (p<0.02), and we found significantly fewer microabscesses in C3H/HeN mice than in BALB/c mice (p<0.005). In supernatants from P. aeruginosa antigen and concanavalin A‐stimulated spleen cells from the two strains of mice, the interferon‐ (IFN‐) γ levels were higher, whereas IL‐4 levels were lower in C3H/HeN mice than in BALB/c mice. The implications of these findings for CF patients with chronic P. aeruginosa lung infection are discussed.