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An in vitro cellular system for generation of AA‐amyloid
Author(s) -
PALM M.,
NIELSEN E. HOLM,
SVEHAG S.E.
Publication year - 1997
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1997.tb05059.x
Subject(s) - amyloid (mycology) , congo red , in vitro , amyloidosis , thioflavin , chemistry , staining , cell culture , biochemistry , microbiology and biotechnology , biology , pathology , medicine , alzheimer's disease , inorganic chemistry , genetics , disease , organic chemistry , adsorption
Different conditions for establishing a cell culture system for generation of AA‐amyloid were investigated. The most effective system was based on peritoneal macrophages from CBA/J mice that had received repeated injections of Hammersten casein, with subsequent cultivation of the cells at high density, high levels of acute phase serum, and neutral pH. Staining with Congo red, thioflavin T, and anti‐AA revealed amyloid‐like structures associated with macrophage clusters. The structures increased in number and size from day 2 to 6 of cell cultivation. The concentration of apoSAA in the culture medium fell markedly in the amyloid‐producing cell cultures, while the SAP concentration was not reduced. The described cell culture system can be useful in studies of the influence of chaperone molecules and other factors on the formation and degradation of amyloid fibrils.